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International Journal of Veterinary Science & Research

Volume 1, Issue 1, 2015
Mcmed International
International Journal of Veterinary Science & Research
Issn
XXX-XXXX (Print), XXXX-XXXX (Online)
Frequency
bi-annual
Email
editorijvsr@mcmed.us
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Abstract
Title
CHARACTERISATION OF STAPHYLOCOCCUS AUREUS OF CATTLE CLINICAL MASTITIS MILK ORIGIN FOR COAGULASE AND HAEMOLYSIN PROPERTIES
Author
V. Khichar and A.K.Kataria*
Email
akkataria1@rediffmail.com
keyword
Cattle, Coagulase, Haemolysins, Mastitis, S. aureus.
Abstract
In the present investigation 28 S. aureus isolates obtained from milk collected from matitic Holstein-Friesian crossbred and Rathi cattle of various parities confirmed by 23S rRNA based genotyping were studied for their coagulase and haemolysin properties. The coagulation reaction was evaluated against plasmas from 10 species of animals and from human in which the overall strongest coagulation reaction was recorded with plasma from rabbit followed by buffalo, cattle, camel, human, goat, sheep, dog, horse, chicken and pig in descending order.All the isolates were haemolytic on sheep blood agar of which 13 isolates showed both α- and β-hemolysis and 15 isolates produced only β-hemolysis which exhibited the phenomenon of Hot-cold lysis. The titre of -haemolysin from H-F isolates ranged between 1:40 and 1:1280, whereas for Rathi isolates it ranged between 1:160 and 1:2560. The titre of -haemolysin was much less for isolates both from H-F (between 1:5 and 1:30) and Rathi (between 1:5 and 1:60) cattle.
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