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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
VISUAL DETECTION OF MELAMINE USING SILVER NANOPARTICLES
Author
Swarnali Maiti, Gadadhar Barman and Jayasree Konar Laha*
Email
j.laha@yahoo.co.in
keyword
Melamine; Silver nanoparticles; Gallic acid; Colorimetric probe.
Abstract
Here we are reporting a new homogeneous assay for detection of melamine using silver nanoparticle synthesized and functionalized by Gallic acid (2, 4, 6-trihydroxy benzoic acid). Various concentrations of melamine were added to test the linearity and sensitivity of the method. The detection of melamine was based on changes in absorbance resulting from aggregation of silver nanoparticles induced by melamine and thereby results in their yellow to greenish color change. A new peak at around 625 nm, in addition to the peak of the AgNP at 415 nm was observed. With the increase of concentration of melamine, the absorbance at 415 nm peak decreased and that of 625 nm increased gradually. The calibration curve obtained from the ratio of the absorption coefficients of these two peaks (Ex 625 / 415 versus concentration of melamine enables one to estimate quantitatively the amount of melamine present in water at ppm levels. The assay relies on the fact that melamine can induce aggregation of silver nanoparticles. The presence of melamine in raw milk can be determined by monitoring with the naked eye or a UV-Visible spectrophotometer. The proposed method is a promising mean for on-site screening of melamine adulterant in raw milk without costly instruments.
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