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European journal of molecular biology and biochemistry

Volume 11, Issue 1, 2024
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
MICROBIAL PRODUCTION OF XYLANASE (EC 3.2.1.8) ENZYME USING BACILLUS SUBTILIS ISOLATED FROM GARDEN SOIL
Author
M.K.Vadivazhagi
Email
mkvadivazagi@gmail.com
keyword
Xylanase, Bacillus subtilis, Enzymes, optimization.
Abstract
Xylans are polysaccharides almost as ubiquitous as cellulose in plant cell walls. In the present study xylanase producing Bacillus subtilis was isolated from garden soil using biochemical microbiological techniques; the isolates were subjected to xylan degradation. The influence of carbon, nitrogen sources, pH, temperature, NaCl, incubation and agitation in the enzymes production was characterized. Produced xylanase enzyme was partially purified by ammonium sulphate precipitation method. The optimum pH, temperature, additives, and carbon and nitrogen sources were as follows 8.0, 32°C, calcium chloride, xylan, and yeast extract respectively.
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