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International Journal of Agricultural and Forestry Sciences

Volume 1, Issue 1, 2016
Mcmed International
International Journal of Agricultural and Forestry Sciences
Issn
XXX-XXXX (Print), XXXX-XXXX (Online)
Frequency
bi-annual
Email
editorijafs@mcmed.us
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Abstract
Title
MICROBIAL PRODUCTION OF XYLANASE (EC 3.2.1.8) ENZYME USING BACILLUS SUBTILIS ISOLATED FROM GARDEN SOIL
Author
K. Saranya and M.K.Vadivazhagi *
Email
mkvadivazagi@gmail.com
keyword
Xylanase, Bacillus subtilis, Enzymes, optimization.
Abstract
Xylans are polysaccharides almost as ubiquitous as cellulose in plant cell walls. In the present study xylanase producing Bacillus subtilis was isolated from garden soil using biochemical microbiological techniques; the isolates were subjected to xylan degradation. The influence of carbon, nitrogen sources, pH, temperature, NaCl, incubation and agitation in the enzymes production was characterized. Produced xylanase enzyme was partially purified by ammonium sulphate precipitation method. The optimum pH, temperature, additives, and carbon and nitrogen sources were as follows 8.0, 32°C, calcium chloride, xylan, and yeast extract respectively.
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