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European journal of molecular biology and biochemistry

Volume 11, Issue 1, 2024
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
OPTIMIZATION OF LIPASE ENZYME ACTIVITY PRODUCED USING BACILLUS MEGATERIUM ISOLATED FROM GARDEN SOIL
Author
K. Padmalochana
Email
kpadmalochana@gmail.com
keyword
lipase, Bacillus megaterium, Enzyme, Optimization.
Abstract
Lipase enzymes are monomeric proteins which are highly exploited due to its vast applications in food, dairy pharmaceutical. The present investigation describes the aim of producing lipase enzyme from olive oil using Bacillus megaterium which is isolated from garden soil. The strain, Bacillus megaterium was identified through biochemical characterization and subjected into lipase production by shake flask fermentations. The enhanced production of lipase enzyme by B. megaterium was achieved by optimized conditions such as carbon source, nitrogen source, substrate, pH, and temperature and incubation time. Result shows that highest lipase activity for Bacillus megaterium was achieved at pH 7 at 30°C for incubation period of 45 hour in the media containing soyameal as carbon source, casein as nitrogen source and gingly oil as substrate source. This optimized condition highly releases the lipase enzymes.
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