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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
PRODUCTION AND OPTIMIZATION OF PROTEASE FROM BACILLUS SUBTILIS ISOLATED FROM AGRICULTURAL SOIL SAMPLE
Author
K. Padmalochana
Email
kpadmalochana@gmail.com
keyword
Protease, Bacillus subtilis, Enzyme, Optimization.
Abstract
Microbial protease enzyme has vast applications in different industries and environmental bioremediation. The objective of this study was to isolate, characterize and optimize the protease producing bacteria. Protease producing bacteria was isolated from garden soil by serial dilution and identified as Bacillus subtilis. Protease production was enhanced by optimizing the fermentation culture conditions like carbon sources and nitrogen sources, NaCl, incubation time, pH, and agitation speed. The Optimum conditions for maximum protease production were achieved by using fructose as carbon Source and urea as nitrogen Source. Maximum protease production was attained at 1 M NaCl concentration. The optimum pH, incubation time and agitation speed for proteolytic activity were 8, 24 h and 250 rpm, respectively. Therefore, this reports showed excellent proteolytic activity of isolated and screened bacteria Bacillus subtilis under optimized physical and chemical conditions.
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