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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
NANOBIOPSY: AN EMERGING INNOVATIVE TOOL FOR INTERROGATING LIVING CELLS
Author
Priya S. Joshi1*, Madhuri Chougule2, Bhagyalaxmi Hongal3, Neha Agnihotri4
Email
sangeetakov@yahoo.co.in
keyword
cancer cell, cell biology, mitochondrial DNA, nano, biopsy, nanotechnology, RNA, robotic.
Abstract
Several approaches have been employed over the years to study tissue architecture and cellular heterogeneity. These have provided insights into cell to cell interaction and circuitry within tissues. Biopsy is one of them and is considered to be the gold standard for definitive diagnosis. Since this procedures are performed on tissues which are removed either from living (biopsy) or dead individuals (autopsy), our ability to study the dynamic function of individual cells in heterogeneous populations in living state has reduced. Thus there is a need to develop a technique which would perform biopsy on tissues & cells without killing them. Robotic nano biopsy has been developed by researchers which is a nano technology based tool to sample living cells without killing them. It uses a glass nano pipette of 50-100 nm in diameter to pierce the cell membrane to extract a volume of 1% of cell and is based on a customized Scanning Ion Conductance Microscope. The tip is so fine that it causes minimal cell destruction. Researchers have used this technique to extract and sequence RNA from individual human cancer cells and mitochondria from human fibroblasts and sequence the mitochondrial DNA. It can also be used to deliver material into cells, opening up ways to re-programme diseased cells. It is a versatile platform for cancer biologists and stem cell biologists who are trying to understand what is happening inside the cell.
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