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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
GROWTH OF Trichomonas vaginalis IN MODIFIED FEINBERG-WHITTINGTON MEDIUM
Author
Fatima Abdelmonim and Elamin Abdelkarim Elamin*
Email
elaminpara72@yahoo.com
keyword
Trichomonas vaginalis, Feinberg-Whittington medium.
Abstract
Trichomonas vaginalis is a protozoan pathogen of the human urogenital tract. A number of different media are now in use for the culture of T. vaginalis, but they are too complex and expensive for ordinary routine work, to that the modification and simplification with available resources in culture medium has a paramount importance. To facilitate using of Trichomonas culture media in routine diagnostic work by modification and simplification of Feinberg-Whittington medium (F.W) with available resources. Sheep liver powder prepared and 8% horse Serum replaced by 5% human serum. Urine samples containing T.vaginales obtained from Bashaier teaching Hospital. The sediment containing parasite was inoculated in prepared medium and incubated at 370C for 24 to 48 hours. The growth was evaluated. T. vaginales and Candida species were growth in modified (F.W) medium. Other flagellates detected in urine samples were converted to cyst after overnight incubation. Conclusion for availability and low cost of modified (F.W) medium, this new medium might be used as an alternative choice for Trichomonas culture method and avoider misdiagnosis with other intestinal flagellates.
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