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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
SDS – PAGE PROTEIN PROFILE OF BOLBITIS SEMICORDATA (MOORE) CHING AND BOLBITIS APPENDICULATA VAR. ASPLENIFOLIA (BORY) SLEDGE FROM SOUTH INDIA
Author
Johnson M*
Email
ptcjohnson@gmail.com
keyword
SDS-PAGE, Protein, Pteridophytes, Bolbitis
Abstract
The present study was aimed to know the inter-specific variation between the two species viz., Bolbitis semicordata (Moore) Ching and Bolbitis appendiculata var. asplenifolia (Bory) Sledge using SDS-PAGE. 500 mg of sporophytes were ground in an ice cold mortar and pestle with 500μl 0.1M phosphate buffer pH 7.0. The resultant slurry was centrifuged at 10,000 rpm for 10 min at 4ºC in cooling centrifuge and the supernatant was stored at 4ºC before use. For separtion of proteins, SDS-PAGE was carried out. A total of 12 bands with multiple regions of activity with varied MW-Rf values were observed in the protein electrophoretic system of Bolbitis species viz., Bolbitis semicordata and Bolbitis appendiculata var. asplenifolia. The SDS-PAGE gel system of Bolbitis failed to show the similarity betweent the studied two species Bolbitis semicordata and Bolbitis appendiculata var. asplenifolia. These protein profiles can be used as a biochemical marker to identify the studied two Bolbitis species.
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