European journal of molecular biology and biochemistry

EFFECT OF GLUCOSE FEED ON CHOLESTEROL, TRIGLYCERIDES AND LIPOPROTEIN CONCENTRATIONS IN PREADIPOCYTES AND ADIPOCYTES: AN IN-VITRO STUDY

Madhukar Saxena*, Dinesh Raj Modi, Sanjay Singh and Anand Prakash

madhukarbio@gmail.com

Adipocytes, Cholesterol, In-vitro, Lipoproteins, Mesenchymal Stem Cells, Preadipocytes.

Adipose tissue is one of the largest cholesterol depots in the body. Adipose cells specialized in energy storage, contain large intracellular triglyceride-rich lipid droplets, are enriched with free cholesterol, and express sterol-regulated transcription factors. In this study, human in vitro cultured human subcutaneous preadipocytes and adipocytes were employed to study the level of cholesterol and lipoproteins. Wharton’s Jelly Mesenchymal Stem Cells were cultured till the accumulation of lipid droplets (Adipocytes). These adipocytes were visualized by Oil Red O staining under phase contrast inverted microscope. Cholesterol and lipoprotein levels were measured by spectrophotometric analysis in both preadipocytes and adipocytes cultured in variable glucose concentration (5-20 mM). The values were plotted using Microsoft excel. Student t-test was used for statistical analysis. The concentration of TC, TGL, LDL and VLDL increase as the glucose concentration increase both in preadipocytes and adipocytes. However concentration of HDL decreases when the glucose concentration increases. All values in each glucose concentration showed highly significant difference except HDL in 5mM and 10mM glucose concentration. The present study examines the aptitude of adipocytes to release their cholesterol, triglycerides and lipoproteins. We show here for the first time that the cholesterol, triglycerides and lipoproteins concentration has been increased due to increase in glucose concentration as the preadipocyte differentiates into adipocytes and showed linear correlation. In conclusion, this study provides new information on cholesterol, triglycerides and lipoproteins release from adipocytes indicates that the post obese complication in in vivo will increase the disease associated with lipid metabolism.