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American Journal of Oral Medicine and Radiology

Volume 5, Issue 2, 2018
Mcmed International
American Journal of Oral Medicine and Radiology
Issn
XXX-XXXX (Print), 2394 - 7721 (Online)
Frequency
bi-annual
Email
editorajomr@mcmed.us
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Abstract
Title
GENDER-WISE PREVALENCE OF STREPTOCOCCUS MUTANS SEROTYPES E, F AND K IN SOUTH INDIAN SCHOOL CHILDREN
Author
1V. Arun Prasad Rao and 2Ravi David Austin
Email
apraprapr@gmail.com
keyword
Dental Caries, Biotypes, Polymerase Chain Reaction, DMFT/dmft, Caries active, Caries free.
Abstract
Dental caries is widely prevalent disease affecting children worldwide. Streptococcus mutans (SM) is the single most common microorganism implicated in the initiation and progress of dental caries. The specific serotypes of SM are c, e, f and k. Serotype c is reported to be most commonly found in the human oral cavity followed by serotypes e, f and k. Currently no data is available on gender-wise distribution of SM serotypes in children. The present study highlights the prevalence of lesser known SM serotypes e, f and k in 6 to 12 years south Indian school going caries active (CA) and caries free (CF) boys and girls. Thirty five boys and 25 girls in the CA (DMFT/dmft≥5) group and 28 boys and 12 girls in the CF (DMFT/dmft=0) group participated in the present study. The prevalence of SM was identified in unstimulated saliva samples by Polymerase Chain Reaction (PCR) amplification method using primers that anneal and amplify a segment of SM-specific GTFB gene. SM specific bands were identified by gel analysis confirmed by direct sequencing. Samples positive for SM were analysed for presence of serotypes e, f and k using primers and PCR techniques. The prevalence of serotype k was found to be the highest in CA and CF boys (31.4% and 18.51%) and girls (36% and 33.3%) followed by serotypes e and f in CA boys and girls and f and e in CF boys and girls. Different combinations of serotypes e, f and k were found in boys and girls of CA and CF groups. The differences between the prevalence of SM serotypes between boys and girls in both CA and CF groups were statistically non-significant.
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