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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
SIMPLE AND RAPID PROTOCOL FOR PECTINASE ZYMOGRAPHY
Author
Abhijeet Singh1, M.K. Upadhyay 2, H.N. Verma 2 and Devendra Jain*3
Email
devendrajain@mpuat.ac.in
keyword
Pectinase, Zymography, Ganoderma, Isozyme.
Abstract
Differentiation of various subgroups of fungi is one of the major challenges for fungal taxonomist. As morphological and cultural characteristic sometime fails to recognize subgroups in a genera or within a species. Therefore paramorphological criteria have attracted the attention of a large group of researcher’s world over. Zymogrpahy of extracellular Pectinase isozyme is now considered to be one of the better tool not only for differentiation of fungi but also for other organisms which are using pectin as sole carbon source. The present study is an attempt to distinguish some morphologically similar but ecologically variant isolates of indigenous Ganoderma lucidum by using simple and rapid pectinase zymography.
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