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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
IN VITRO ANTI-OXIDANT ACTIVITY OF FUCOIDAN EXTRACTED FROM PADINA DISTROMATICA HAUCK (BROWN SEAWEED) FROM HARE ISLAND, THOOTHUKUDI, TAMIL NADU, INDIA.
Author
John Peter Paul J*
Email
johnarock2008@yahoo.com
keyword
Brown seaweed, Padina distromatica, Fucoidan, Anti-oxidant, DPPH, CUPRAC.
Abstract
The aim of the present study was to assess the antioxidant activity of fucoidan extracted from Padina distromatica Hauck in Hare Island, Thoothukudi, Tamil Nadu, India. Free radical scavenging activity was evaluated using 1,1-diphenyl2-picryl hydrazyl (DPPH) free radical method and a reducing power by Cupric Reducing Antioxidant Capacity (CUPRAC) assay. The percentage of scavenging activity of DPPH by fucoidan at 100μg, 200μg, 300μg, 400μg and 500μg were 40.43, 59.68, 68.47, 87.32 and 94.33%, respectively. At a concentration of 100μg, 200μg, 300μg, 400μg and 500μg of fucoidan, the absorbance were 0.302, 0.317, 0.369, 0.395 and 0.427 respectively. These results similar to those obtained from the DPPH assay in which 500μg showed the highest total antioxidant capacity, followed by 400μg, 300μg and 200μg, and lastly 100μg. The results showed that both DPPH scavenging activity and absorbance were increased when the concentration of fucoidan was also increased. Vitamin C, a strong anti-oxidant was also used as control, and the anti-oxidant potential was compared to all tested samples.
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