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American Journal of Oral Medicine and Radiology

Volume 5, Issue 2, 2018
Mcmed International
American Journal of Oral Medicine and Radiology
Issn
XXX-XXXX (Print), 2394 - 7721 (Online)
Frequency
bi-annual
Email
editorajomr@mcmed.us
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Abstract
Title
THE VIABILITY TESTING OF GIARDIA LAMBLIA CYST
Author
Hiba Nasir and Elamin Abdelkarim Elamin*
Email
elaminpara72@yahoo.com
keyword
Giardia lamblia, Viability.
Abstract
The parasite Giardia lamblia is a very simple eukaryote that is a unicellular protozoan parasite causes giardiasis, which is prevalent throughout the world. Patients complain of several symptoms as gastroenteritis that manifests itself with severe abdominal pain and diarrhea .This study was designed to determine the impact of different environmental conditions on the viability of Giardia lamblia cysts. One gram of faeces was dissolved in 35ml of Normal Saline. Giardia lamblia cysts were counted by using Haemocytometer and were exposed to different temperature as follows 100, 70, 60, 50, 40, 25, 15, 4, -20, Each of them was left for three different incubation periods as follows 15min,3 0min, 60min, Viability testing was done for the samples by using super-vital stains. Result shows that the study found that the cysts survived for 15min when exposed to temperatures 70oC, 60oC, 50oC, 40oC, 37oC, 25oC, 15oC, 4oC, and -20oC. While they survived for 30min is when exposed to temperatures 60oC to 20-oC. Furthermore they were survived for 60min when exposed to temperatures from 60oC to 4oC . This study concludes that Giardia lamblia cyst cane survive longer time at temperatures between 60 and 4oC and cannot resist 100oC.
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