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Acta Biomedica Scientia

Volume 1, Issue 2, 2013
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
COMPARATIVE STUDY BETWEEN PCR, ICT AND MICROSCOPY IN DIAGNOSE OF PLASMODIUM FALCIPARUM
Author
Hana Ibraheem and Elamin Abdelkarim Alamin*
Email
elaminpara72@yahoo.com
keyword
Plasmodium species, Direct microscopy, Guanidine Hydrochloride Extraction Method
Abstract
Microscopic detection of Plasmodium species has been the reference standard for the diagnosis of malaria for more than a century. However, maintaining a sufficient level of expertise in microscopic diagnosis can be challenging, particularly in non-endemic countries. The main objective of this study was to compare the performance of nested PCR with expert microscopy and rabid test as a means of detecting Plasmodium faciparum parasites in different hospitals of Khartoum state. Thirty venous blood samples was collected in to a dry EDTA container and preserved at refrigerator for subsequent processes. Direct microscopy was carried out by preparing thin and thick blood films and staining with Giemsaꞌs stain, and then ICT was carried out. DNA was extracted from samples using guanidine hydrochloride extraction method to detect P.falciparum DNA. With comparison PCR as gold standard method, there was high sensitivity and specificity detected by microscopy 50%, 54% respectively. In the SD® ICT assay there was sensitivity (25.5%) and low specificity (56%). ICT diagnostic technique cannot be recommended as a sensitive and specific tool for malaria diagnosis. PCR appears to be a useful method for detecting Plasmodium parasites.
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