European journal of molecular biology and biochemistry

ANTIBACTERIAL ACTIVITY OF ANNONA SQUAMOSA L. AGAINST STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS PYOGENES

Deepak Panwar* and R.P. Bhatt

deep.pw.@gmail.com

MIC, MBC, Annona squamosa, Staphylococcus aureus, Streptococcus pyogenes.

Evaluated the antibacterial effect of aqueous and ethanolic extract of the leaf, bark of Annona squamosa L. against Staphylococcus aureus and Streptococcus pyogenes was analyzed in this study. All the selected strains were previously characterized. Aqueous and ethanolic extract of the leaf, bark of Annona squamosa L. were prepared with help of soxhlet unit. Further, evaluated the antimicrobial activity of these extract were analyzed against S. aureus and S. pyogenes. Aqueous and ethanolic extract of the leaf and bark of Annona squamosa leaf showed significant antibacterial activity against Staphylococcus aureus and Streptococcus pyogenes. The 15 μg/ml aqueous extract of leaf of the Annona squamosa showed 1.66, 24.02% antimicrobial activity against Staphylococcus aureus and Streptococcus pyogenes respectively as compared to the control. Similarly, 15 μg/ml ethanolic extracts of Annona squamosa leaf showed more inhibition zones (23.16 and 21.34 %, respectively) against Staphylococcus aureus and Streptococcus pyogenes. The 15μg/ml aqueous extract of bark of the A. squamosa showed 51.88, 78.77% more inhibition zone against Staphylococcus aureus and S. pyogenes respectively whereas 15 μg/ml ethanolic extract of bark of the A. squamosa exhibited 59.02, 99.45% more inhibition zone against Staphylococcus aureus and S. pyogenes respectively as compared to 15μg/ml norflox (control drug). Aqueous and Ethanolic extract of bark of Annona squamosa showed 5μg/ml MIC and 10μg/ml MBC against Staphylococcus aureus and Streptococcus pyogenes. Ethanolic extract of leaf of Annona squamosa showed 5μg/ml MIC but aqueous extract of leaf of Annona squamosa showed 10μg/ml MIC.