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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF TRICHODERMA ISOLATES OF TAMILNADU
Author
K.Saravanan*, M.Anitha, N.Rajabairavi, R.Prabhavathy, A.Kalirajan, R.Chandrasekaran, V.Vijayalakshmi, B.Aarthy, T.Thilagam
Email
snrsaro88@gmail.com
keyword
Conidia, Phialide, T.atroviride, T.harzianum, T.viride.
Abstract
Fifteen isolates of Trichoderma viride, Trichoderma atroviride and Trichoderma harzianum obtained from rhizosphere soil of plantation crops, forest soil and agricultural field of Tamilnadu region were studied using morphological characters, molecular characterization using Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats-Polymerase Chain Reaction. The morphological characters were based on the following characters included here viz., colony growth rate (after 7days in cm) at 28±1ºC, colony colour, reverse colony colour, colony edge, culture smell, conidiation, mycelia form, mycelia colour, conidiophores branching, phialide disposition, phialide shape, conidial shape, conidial wall etc. The isolates were grouped into three distinct clusters of T.harzianum, T.viride and T.atroviride. The similarity ranged from 50 to 91%. The maximum genetic variation in the T. viride group was observed between the isolates T4 and T7 (90%). In T. harzianum cluster variation ranged between T8 and T12 (88%). Followed by T3 and T5 (79%) in the T. atroviride group. On the other hand maximum genetic variation of 51 % was observed between the cluster I and followed by 55% between cluster II and cluster III.
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