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International Journal of Advances In Case Reports

Volume 11, Issue 1, 2024
Mcmed International
International Journal of Advances In Case Reports
Issn
XXX-XXXX (Print), 2349 - 8005 (Online)
Frequency
bi-annual
Email
editorijacr@mcmed.us
Journal Home page
http://mcmed.us/about/ijacr
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Abstract
Title
EVALUATION OF RAPID IMMUNO-CHROMATOGRAPHY TEST NS1/ Ig M/Ig G, NS1 ANTIGEN AND Ig M CAPTURE ENZYME LINKED IMMUNO-SORBENT ASSAY FOR DETECTION OF DENGUE VIRUS
Author
Narayan Shrihari*1, Krishna S2 and Ramakrishna K3
Email
1Associate Professor, Department of Microbiology,
keyword
Dengue infection, Rapid immuno-chromatography test (Rapid ICT) and enzyme linked immuno-sorbent assay (ELISA).
Abstract
Rapid diagnosis of Dengue virus infection is essential for the patient management. The rapid immuno-chromatography test (Rapid ICT) and enzyme linked immuno-sorbent assay (ELISA) for dengue NS1 antigen and Ig M antibody. ELISA is giving more accurate result as compared to Rapid ICT. The Objective is to evaluate and compare rapid ICT and both ELISA for dengue virus infection. The laboratory records of clinically suspected dengue patients from January to August 2014 were analyzed retrospectively and results of NS1 antigen and antibody of dengue virus tested by rapid ICT and ELISA respectively and we compared the accuracy of both the tests. Both the rapid ICT and ELISA (NS1 and IgM) tests were performed by following the manufacturer’s instructions. The NS1 capture ELISA showed excellent sensitivity as compared to rapid ICT. The rapid test detected NS1 antigen and 258 serum samples were positive out of 1106(23.33%). The ELISA test detected NS1 antigen and 146 serum samples were positive out of 258(56.59%). The ELISA IgM captured and 223 serum samples were positive out of 1482(15.05%).The present study emphasizes the continuous sero-epidemiological surveillance for the effective dengue virus infection control programme. These tests should be a useful aid in confirming the clinical diagnosis of dengue virus infection. The rapid test will be particularly valuable in peripheral health setting while the ELISA has a place in central testing Laboratories.
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