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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
A COMMON METHOD APPLY FOR DNA EXTRACTION FROM LEAVE OF SOME IMPORTANT PLANTS SPECIES
Author
K. Khasdeo1, Sonam Chouhan1, Alice R. Tirkey1, Chitra Pandagre1, Neha Akhande1, Anil Kumar*1, Asha D Lazras2 and B.L. Jharia3
Email
kmr.nano@yahoo.com
keyword
SDS, DNA, TBE, UV-spectroscopy.
Abstract
In this investigation of a large number of samples such as Fresh leaves obtained from different tree species viz., Azadirachta indica, Bixa orellana L, Elaeocarpus ganitrus, Emblica officinalis, Mimosa pudica, Pongamia pinnata, Rosa rubigenos, Saraca asoca, require faster methods of Lin et al at SDS based applied for DNA extraction and purification method which can provide huge amount of high quality DNA determined by horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 100V and purity measured on 260/280nm of ratio near to 1.8 by UV-spectrometer.
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