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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
IMMUNOSTIMULANT EFFECT OF ON Eichhornia crassipes (MART.) SOLMS AGAINST P. fluorescens INFECTED INDIAN MAJOR CARP Labeo rohita (HAM.)
Author
G. Dineshkumar1, R. Rajakumar1, P. Mani2*
Email
master.maniji@gmail.com
keyword
P. fluorescens, Eichhornia crassipes Labeo rohita, Bacterial agglutination assay, Immunostimulant.
Abstract
Fish is one of the cheapest and promising sources of animal protein .People can easily digest 93.2% and 93.7% of fish protein and fat respectively .Now a days, the bacterial infection acts as an important limiting factor for fish culture. Especially, the infection of Pseudomonas fluorescens bacterium has lead to great economic loss for fish culture business. The uncontrolled and repeated use of antibiotics to treat bacterial infection lead to development of antibiotic-resistant pathogens. To overcome this problem, antibiotics from plants resources cannot produce resistant bacterium and it can be frequently used to control fish diseases. Medicinal plants are used for the formation of drugs and these plants are used traditionally to cure various diseases. These medicinal plants contain some phytochemical active compounds such as flavonoids, alkaloids, tannins, and phenols etc., which shows Immunostimulant activity against the pathogens. Hence, the present study investigated the immuno stimulatory effect of Eichhornia crassipes against P. fluorescens .The plant leaves extract E. crassipes treated fish group showed no mortality. The bacterial agglutination assay of the plant extract treated fish exhibited earlier and more bacterial agglutination and the respiratory burst activity was also high. Both acid and alkaline phosphatase was reduced at the end of the experiment and the catalase was increased. Heamatological parameters were also elevated. It is concluded that 30 ppm of E. crassipes plant leaves extract is more effective to defend P. fluorescens. Thus E. crassipes acts as an immunostimulant to enhance the activity of fish.
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