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European journal of molecular biology and biochemistry

Volume 11, Issue 1, 2024
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
IN-SILICO PREDICTION AND FUNCTIONAL ANALYSIS OF SALT STRESS RESPONSIVE GENES IN MAIZE (Zea mays)
Author
Jyotika Bhati1, Pavan Kumar Chaduvula1, Ruchi Rani2, Sanjeev Kumar*3 and Anil Rai4
Email
sanjeevk@iasri.res.in
keyword
Maize, Salt Stress, Functional Annotation, Gene Prediction and Promoter Analysis.
Abstract
Maize is frequently impacted by salt stress condition. Therefore, the present work emphasises on reconstruction and annotation of salt stress genes. In order to identify genes responsible for salt stress in Maize 116258 expressed sequence tags (ESTs), expressed in salinity stress condition, were mined from the different web recourses. The downloaded ESTs were clustered and assembled into 11042 contigs. Biological functions were obtained only for 6448 out of 11042 contigs through Gene Ontology (GO). The remaining contigs were used for reconstruction, validation and annotation of salt stress genes. These contigs were mapped on to Maize genome and full length gene sequences were designed. These designed candidate genes were further validated by means of promoter analysis. The study claims the possible involvement of the predicted genes in salt stress mechanism and may be useful in molecular breeding programme in Maize salinity research.
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