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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
IN VITRO SYNERGISTIC EFFECT OF ANTIBACTERIAL ACTIVITY OF PIPER NIGRUM AND RAUWOLFIA SERPENTINA AGAINST CERTAIN PATHOGENS
Author
Vishal Kumar Deshwal*1, Kavita vig2 and Pawan Kumar Tripathi3
Email
vishal_deshwal@rediffmail.com
keyword
Piper nigrum, Rauwolfia serpentina, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus warneri, Salmonella typhi, Klebsiella pneumoniae Norfloxacin, Synergism, Antimicrobial activity.
Abstract
The medicinal plants, Piper nigrum (fruit) and Rauwolfia serpentina (Root) was tested for their synergistic effect as antibacterial agents against six characterized pathogens Escherichia coli, Pseudomonas aeruginosa, Staphylococcus warneri, Salmonella typhi, Klebsiella pneumoniae, Shigella dysentriae. The aqueous, ethanol and chloroform extracts of fruit of Piper nigrum and Rauwolfia serpentina were Preparation by standard methods. In a ratio of 50:50 (20μ/ml of each), the mix of three solvent extracts of these two plants was prepared. Of three different solvent extracts, total of three treatments (T2a, T2b, T2c) were prepared. The results showed that the first treatment (T2a) i.e. mix of aqueous extract of the two plants gave the largest inhibition zone as compared two other two solvent mix against Staphylococcus warneri (17.2mm), followed by chloroform mix (third treatment/ T2c) which again gave largest inhibition zone against Staphylococcus warneri (17.0mm). The first treatment (T2a) showed very good inhibition effect against Shigella dysentriae (16.2mm) which was seen to be more than individual plant extracts as well as standard antibiotic Norfloxacin (13.9mm). Then treatment two (ethanol extract mix/ T2b) gave largest inhibition zone against Staphylococcus warneri (16.8mm) and all were slightly less than the used standard antibiotic Norfloxacin (17.8mm). The smallest inhibition zone was against Escherichia coli (14.8mm) by treatment three (chloroform extract mix).
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