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Acta Biomedica Scientia

Volume 1, Issue 2, 2013
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
UV-VIS AND HPLC STUDIES ON PADINA GYMNOSPORA (KUTZING) SONDER AND PADINA TETRASTROMATICA HAUCK
Author
Johnson M*, Nellaiammal alias Suki R, Janakiraman N
Email
ptcjohnson@gmail.com
keyword
Phytochemistry; Padina gymnospora; Padina tetrastromatica; UV-VIS; HPLC; Chemotaxonomy.
Abstract
The present study was aimed to investigate the phytochemical constituents and physico-chemical parameters present in the seaweeds Padina gymnospora (Kutzing) Sonder and Padina tetrastromatica Hauck. Preliminary phytochemical screening was carried out by the method described by Harborne. The extracts of P. gymnospora and P. tetrastromatica were scanned in the wavelength ranging from 200-1100 nm by using Shimadzu Spectrophotometer. HPLC method was performed on a Shimadzu LC-10 AT VP HPLC system, which was equipped with a model LC-10AT pump, UV-Vis detector SPD-10AT, Rheodyne injector fitted with a 20 μL loop and auto injector SIL-10AT. The qualitative phytochemical screening revealed the presence of alkaloids, steroids, phenolic groups, saponins, tannins, flavonoids, glycosides, proteins and sugars. The results of ash analysis discovered the existence of sulphur, calcium, magnesium, iron, phosphorous and chlorine in all the extracts of P. gymnospora and P. tetrastromatica. The fluorescence analysis results showed different colours both under ordinary and UV light. The UV-VIS fingerprint profile of various extracts of P. gymnospora and P. tetrastromatica produced different peaks which confirmed the presence of variety of metabolites. The HPLC profile showed the compound separated at a retention time of 2.657 was identical in both the species P. gymnospora and P. tetrastomatica. The generated data may be useful in suggesting chemotaxonomical interrelationship of two species. Further advanced studies are required for structural elucidation and identification of compounds detected in P. gymnospora and P. tetrastomatica.
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