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European journal of molecular biology and biochemistry

Volume 12, Issue 1, 2025
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
EXPLORING THE ANTIOXIDANT, CYTOTOXIC, AND BACE-1 INHIBITION POTENTIAL OF OCHNA SQUARROSA ROOT BARK: IN VITRO AND IN SILICO INSIGHTS
Author
Dr. Vijayalakshmi N
Email
Vijayaotri@gmail.com
keyword
Oxidant; BACE-1; docking; O. squarrosa; inhibition; cytotoxicity
Abstract
Alzheimer's disease (AD) is marked by amyloid beta peptide (A?) accumulation in highorder association brain regions, and growing evidence suggests that A? plays an essential early role in AD pathogenesis. The ethanol extract of the root bark was prepared and used to identify the antioxidant activity by DPPH and nitric oxide scavenging assay. The cytotoxicity of the extract was identified by brine shrimp lethality assay. The molecular docking was performed with the phytoconstituents reported in the root bark of O.squarrosa. The DPPH and nitric oxide assay showed a maximal inhibitory potential of 68.62 % and 73.14 % at 1000 µg/ml. Also, the IC50 of the extract was found to be 307.1 µg/ml (DPPH) and 247.5 µg/ml (nitric oxide). The lethality assay was carried out for 24hrs and 50 % death was observed at 1000 µg/ml. The molecular docking study revealed that the phytoconstituent 3’,4’-Dihydroxyfurano [3’’,2’’: 6,7] flavone showed high docking score with a mechanism-based inhibition of BACE-1 protein. These data suggest that the phytoconstituents may have a BACE-1 inhibition potential and further in-vitro and in-vivo screening is required to confirm the inhibitory potential.
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