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European journal of molecular biology and biochemistry

Volume 12, Issue 1, 2025
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
ROLE OF CS21 TYPE IV PILI IN ADHESION AND VIRULENCE OF ENTEROTOXIGENIC ESCHERICHIA COLI IN EPITHELIAL AND NEONATAL MOUSE MODELS
Author
Lakshmi G N A*
Email
keyword
Enterotoxigenic Escherichia coli, CS21, Type IV pili, Adhesion, Virulence
Abstract
Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal diseases, especially in travelers and infants in developing regions. A key virulence factor of ETEC, the CS21 type IV pili, plays an essential role in intestinal colonization and microcolony formation. However, the exact role of CS21 in ETEC pathogenesis remains not fully understood. Methods: This study aimed to explore the mechanisms of CS21- mediated adhesion using both in vitro epithelial cell models (IPEC-J2 and IPEC-1) and in vivo neonatal mouse models. Quantitative adhesion assays were conducted to compare the adhesion capacity of CS21-expressing ETEC strains and their CS21-deficient mutants. Additionally, the effect of anti-LngA monoclonal antibodies and neuraminidase treatment on adhesion was evaluated. Results: The results demonstrated that CS21-expressing ETEC strains exhibited significantly higher adhesion to epithelial cells compared to CS21- deficient mutants. Adhesion was inhibited by anti-LngA monoclonal antibodies and neuraminidase treatment, indicating a role for CS21 in the adhesion process. In vivo, CS21- expressing ETEC strains showed enhanced virulence in neonatal mice, supporting the importance of CS21 in gut colonization and pathogenicity. Conclusion: This study highlights CS21 as a critical virulence factor in ETEC pathogenesis. These findings provide valuable insights into the molecular mechanisms of ETEC adhesion and suggest CS21 as a potential target for novel therapeutic strategies to combat ETEC-related infections.
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