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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
ISOLATION OF AMYLASE PRODUCING BACTERIA AND OPTIMIZATION OF GROWTH CONDITIONS
Author
Nayana M1, Nisha P1*and Ardra Asokan2
Email
nishapthirumeni@gmail.com
keyword
Amylase, Micrococcus, Polluted water, Bioparameters, DNSA Method.
Abstract
Starch degrading bacteria isolated from polluted water samples and screened the isolates by starch agar base plate method. More potent bacteria were identified as Micrococcus sp VR. By morphological and biochemical characters based on Bergey’s manual determinative bacteriology and DNA sequencing. Effect of bio parameters on enzyme production and enzyme activity by Dinitrosalicylic acid method were studied by adjusting the incubation temperature at 270C and 370C, incubation time (24, 48, 72hrs), production medium pH range from 6 to 8 (6, 6.2, 6.4, 6.6, 6.8, 7, 7.2, 7.4, 7.6, 7.8 and 8) and different concentration of starch (0.5, 1, 1.5, 2 & 2.5%) and optimize growth conditions. Maximum amylase activity of VR was 0.9942 IU/L at 1% of starch, 370C, and 24 hr of incubation and at pH 7.2.
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