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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
POST-SURGERY OR ANESTHESIA, MONONUCLEAR CELLS EXPRESS AND RELEASE HIGH MOBILITY GROUP BOX 1 (HMGB1) PROTEIN
Author
Dr. Bondugula Prathyusha
Email
keyword
High mobility group box 1, neuro-endocrinehormone flux, Stress response
Abstract
During major elective surgery, High mobility group box 1 (HMGB1) proteinv production kinetics were investigated, along with circulating mononuclear cells. Interleukin-6 (IL-6) and HMGB1 were also examined. We enrolled 94 patients scheduled for major abdominal procedures. In addition to midazolam, thiopentone sodium and fentanyl were given to all patients as well as Vecuronium. In total, 6 samples were collected from patients before, after, and 24 hours following surgery, t0: before surgery, t1: immediately following surgery, and t2: 24 hours after surgery. Anti-CD14-coated microbeads were used to purify monocytes, followed by magnetic sorting. HMGB1 cellular localization was determined by flow cytometry and serum HMGB1 release by Western blotting. ELISA was used to measure serum levels of IL-6. HMGB1 concentrations in monocytes and serum were assessed using one-way repeated-measures analysis. At t1, HMGB1 expression was significantly higher in monocytes than at t0; at t2, serum HMGB1 levels increased significantly. As a result of such an increase, cellular HMGB1 was significantly downregulated, suggesting that HMGB1 might be partly produced by monocytes; c) monocytes treated with HMGB1 released IL-6 in vitro; d) at t2, levels of circulating IL-6 were elevated. Surgical/anesthesia trauma can cause monocytes of surgical patients to express HMGB1, suggesting these cells contribute to the production of HMGB1
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