European journal of molecular biology and biochemistry

PHYTOCHEMICAL STUDIES ON POLYGONUM GLABRUM WILLD

Shibila T, Johnson M*, Narayani M and Revathy I

ptcjohnson@gmail.com

UV-VIS, FT-IR, EDS, Histochemistry.

The present study was aimed to explore the biochemical and phytochemical profile of Polygonum glabrum (Willd.). To identify the presence or absence of metabolites like lignin, tannin, chitin, suberin, phenols and poly phenols in the leaves, stem and root of P. glabrum histochemical analysis were performed. Preliminary qualitative phytochemical screening of P. glabrum leaves extract was carried out. To reveal the functional metabolites and elements UV-Vis, FT-IR and Energy Dispensive X-ray Spectrometer analysis were performed. The occurrence and distribution of various metabolites viz., lignins, polyphenols and tannins on various parts of P. glabrum were illustrated. The epidermis and vascular bundles of root and stem showed higher amount of metabolites than cortex. Preliminary phytochemical analysis revealed the presence of steroids, alkaloids, phenolic groups, cardiac glycosides, flavonoids, saponins, tannins and sterols in P. glabrum. The extracts of P. glabrum demonstrated varied spectrum with different absorption and the results of UV-Vis analysis were tabulated. The results of P. glabrum FTIR analysis confirmed the presence of alkynes, alkenes, esters, ethers, aromatics, alkyl halides, aliphatic amines, carboxylic acids, aldehydes, nitro compounds, primary, secondary, tertiary amines and phenols. The EDS analysis revealed the occurrence of four elements viz., carbon, oxygen, chlorine and potassium in the ethanolic extract of P.glabrum. The results of phytochemical and biological analysis showed that the metabolites present in the leaves of P. glabrum can be used to cure various diseases.These spectroscopic profiles will act as pharmacognostic marker to distinguish the medicinally important P. glabrum using relatively simple, cost-effective spectroscopic profile from its adulterants in the pharmaceutical industries.