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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
POLYETHYLENEIMINE-MEDIATED GENE DELIVERY ENHANCES TRANSFECTION EFFICIENCY IN NASAL INFERIOR TURBINATEDERIVED STEM CELLS: IMPLICATIONS FOR REGENERATIVE MEDICINE
Author
Dr. Chandra Kiran
Email
keyword
Stem cells, Polyethyleneimine (PEI), Gene therapy, Non-viral delivery, Nasal inferior turbinates
Abstract
New types of stem cells, derived from the tissues of the nasal inferior turbinates (hTMSCs), hold promise for regenerative medicine applications. These cells can be readily obtained from tissue donated during turbinectomy or conchotomy procedures. In this study, polyethyleneimine (PEI) was investigated as a gene carrier for hTMSCs using a non-viral delivery system. DNA-PEI nanoparticles (NPs) were generated via electrophoresis and characterized for particle size. The cytotoxicity and transfection efficiency of the DNA-PEI NPs were evaluated in hTMSCs through fluorescence imaging, flow cytometry, and MTT assays following a 4-hour treatment. While DNA-PEI NP treatment exhibited some cytotoxic effects, electrostatic interactions improved transfection efficiency. These findings demonstrate the potential of PEI as an efficient DNA carrier for hTMSCs, suggesting their suitability for gene therapy applications in regenerative medicine
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