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Acta Biomedica Scientia

Volume 4, Issue 3, 2017
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
INSULIN EFFECTS ON HUMAN PLEURAL ELECTROPHYSIOLOGY: INSIGHTS FROM COMPARATIVE STUDY WITH PLEURA AND INTERVENTION WITH GLIBENCLAMIDE
Author
Dr. Chandra Kiran
Email
keyword
Diabetes, Glibenclamide, Insulin, Immunity
Abstract
The study aimed to investigate the direct effect of insulin on human pleural electrophysiology, analogous to findings observed in sheep pleura. Utilizing glibenclamide, a hypoglycemic agent, as a glucoward, researchers sought to determine if insulin elicited similar effects in human pleura, the involvement of insulin receptors, and the potential reversibility of these effects by glibenclamide. Human parietal pleural specimens were mounted in a Ussing chamber, and various solutions containing glibenclamide, insulin with anti-insulin antibody, and insulin with anti-insulin receptor antibody were applied. Transmesothelial resistance (RTM) was measured using the RTM method, while immunohistochemistry was employed to detect insulin receptors (IRa, IRb). The study found that anti-insulin and anti-insulin receptor antibodies inhibited insulininduced changes in RTM when applied mesothelially within the first minute. Moreover, glibenclamide effectively eliminated insulin-induced alterations. Immunohistochemistry revealed evidence of IRb and IRa. These findings suggest that interference with insulin receptors leads to electrochemical changes in both humans and sheep pleura, which can be mitigated by glibenclamide intervention
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