TELL : +91 0 99 08 87 07 06

 Mcmed International ®: Largest Journal Publication in world

European journal of molecular biology and biochemistry

Volume 6, Issue 1, 2019
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
Recommend to
A Friend  | A librarian
Purchase
Purchase Issue
Abstract
Title
EXPLORING THE ROLE OF PRIMITIVE STEM CELLS IN RECEPTOR EXPRESSION MANIPULATION FOR CYTOTHERAPY IN APLASTIC ANEMIA: INSIGHTS FROM CHEMOTHERAPEUTIC DRUG EFFECTS ON SCA-1+ HEMATOPOIETIC STEM CELLS
Author
Sreshmitha Manchala
Email
keyword
Hematopoietic stem cells, Aplastic anemia, Chemotherapeutic drugs, Apoptosis, Bone marrow regeneration.
Abstract
All blood cell lineages are derived from hematopoietic stem cells (HSCs) through a process known as hemopoiesis, wherein these stem cells exhibit self-renewal capabilities. As murine HSCs differentiate, their expression of Sca-1, along with other mature cell markers, diminishes, indicating their transition towards a more specialized state. Throughout an individual's lifespan, Sca-1+ HSCs within the bone marrow sustain the pool of stem cells necessary for hematopoiesis. Aplastic anemia, characterized by a decline in the production of healthy blood cells, is believed to stem from a primary deficiency in stem cells. Our laboratory studies have shown that during experimental drug therapy, specifically with chemotherapeutic agents like Busulfan and Cyclophosphamide, Sca-1+ BM-HSCs are significantly impacted. Elevated levels of Caspase-3 activity and Annexin-V positivity in the bone marrow indicate a heightened susceptibility of hematopoietic stem cells to premature apoptosis. This susceptibility is more pronounced in Sca-1bright BM-HSCs compared to Sca-1dim BM-HSCs. Scanning electron microscopy (SEM) reveals a disrupted microenvironment within the marrow, which correlates directly with the decline in cell population and receptor expression. Based on the aforementioned experimental findings, it is proposed that primitive stem cells may have a role in the regeneration of the bone marrow. Manipulating receptor expression for cytotherapy in aplastic anemia patients could potentially leverage these primitive stem cells for therapeutic intervention.
Download Broucher
Back to Top >>>>