Acta Biomedica Scientia

AN INVESTIGATION OF THE BIOCOMPATIBILITY AND IN VITRO EFFICACY OF ANTIBIOTIC-COATED CENTRAL CATHETERS

Dr. Parija Jyothi Prakash

drvrvkk@gmail.com

Anti-biotics, catheters, blood stream infections, gendine-coated catheters

It is possible that antimicrobial peripherally inserted central catheters, also known as central catheters, will lower the rate of bloodstream infections caused by central lines. This is because piccs, which are also known as central catheters, are inserted into the peripheral arteries. Using an in vitro biofilm colonization model, we evaluated the antimicrobial efficacy of a novel gendine-coated (combination of chlorhexidine [chx] and gentian violet [gv]) picc. In this study compares piccs that have been pretreated with minocycline/rifampin (m/r) and cholex. Rabbits were used in the research for this model. For a period of four days, rabbits were implanted with peripherally inserted central catheters (piccs) in their jugular veins. These catheters were either coated with gendine or left uncoated as a control. At the end of the four-day period, a histopathological analysis was performed, A liquid chromatography-mass spectrometry measurement was done at various time points throughout the experiment to determine the concentration of chx and gv in the blood. To test the antimicrobial, the strains of methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, Pseudomonas aeruginosa, Escherichia coli, Acinetobacterbaumannii, Enterobacter cloacae, candida albicans, and candida glabrata were isolated from clinical samples. In rabbits, gendine-coated piccs demonstrated a significantly lower rate of thrombosis and inflammation following simulated intravascular indwellings of 24 hours and 1 week compared to uncoated controls. This was the case when comparing the two groups of rabbits. During the entirety of the research project, not a single instance of gv was discovered in any of the blood samples; on the other hand, chx was discovered in extremely low concentrations. From 24 hours to 1 week, all pathogens were completely prevented from adhering to the gendine-coated piccs (p 0,001), whereas it was not prevented on the m/r-treated, chx-treated, or control piccs. Piccs that had been coated with gentine were found to be very effective at preventing the formation of biofilm by multidrug-resistant bacterial and fungal pathogens. This was demonstrated by the fact that no biofilm was found on the piccs. Piccs with a gendine coating were found to be biocompatible when used within the intravascular environment. In addition, from pharmacokinetic testing, it was determined that chx and gv were well within safe limits during acute systemic exposure from the gendine-coated catheters. This was determined by the fact that the acute exposures were measured in milligrams per kilogram of body weight. This was proven by the fact that the levels were located well within the acceptable ranges for safety