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Acta Biomedica Scientia

Volume 4, Issue 3, 2017
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
ASSESSMENT OF GENETIC DIVERSITY OF ANTI-CANCER DRUG YIELDING PLANT ANDROGRAPHIS PANICULATA NEES FROM THE WESTERN GHATS OF TAMIL NADU USING ISSR MARKER
Author
T. Leon Stephan Raj
Email
leostephanraj@gmail.com
keyword
Genetic Diversity, Western Ghats, Andrographis paniculata, ISSR-PCR analysis
Abstract
In the present investigation the genetic diversity of anti-cancer drug yielding plant Andrographis paniculata Nees collected from the Western Ghats of Tamil Nadu was carried out using ISSR- PCR makers. After screening twenty ISSR primers, 10 intense and reproducible bands produced primers were selected for the study. ISSR markers amplified products produced a total of 50 loci in ten primers at ten different populations of Andrographis paniculata. High genetic variation at species level was observed in the present investigation with the recording of 96 percentages of polymorphic bands (PPB) and with the 48 number of polymorphic loci. The mean value of overall observed and effective number of alleles were 1.96 and 1.6435. Nei’s gene diversity and Shannon information index were 0.3697 and 0.5446. The mean value of genetic diversity within population (Hs) and the total genetic diversity (Ht) of the plant was recorded as 0.1120 and 0.3697 respectively. The observed genetic differentiation among populations (Gst) was 0.6970 and the estimate of gene flow among populations was 0.2174. The unweighted pair-group method using arithmetic average method of dendrogram was constructed using MEGA software. The genetic identity and difference among the populations were displayed in different clusters. The superior genotype was selected based on high genetic variability with more individual percentage of polymorphism
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