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Acta Biomedica Scientia

Volume 4, Issue 3, 2017
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
ANTIBACTERIAL ACTIVITY, IN VITRO ANTIOXIDANT POTENTIAL AND GC-MS CHARACTERIZATION OF METHANOLIC EXTRACT OF Tricholoma equestre, A WILD MUSHROOM FROM SOUTHERN WESTERN GHATS, INDIA
Author
Ragupathi. V , Stephen. A , Arivoli. D and Kumaresan S
Email
kumareshrkm@yahoo.co.in
keyword
Tricholoma equestre, antibacterial activity, in vitro antioxidant potential, GC-MS.
Abstract
The present study was conducted to assess the antibacterial activity by disc diffusion technique, antioxidant potential by Nitric oxide scavenging as well as DPPH radical scavenging assays and GC-MS characterization of methanolic extract of Tricholoma equestre collected from southern Western Ghats, India. Methanol extract showed the best activity against Enterococcus faecalis, Eggerthella lenta and Vibrio parahaemolyticus than the standard antibiotic amoxicillin. The methanolic extracts of T. equestre with two complementary test systems, namely DPPH free radical scavenging and Nitric oxide scavenging activity showed inhibition EC50 µl values of 35.43% and 10% μg/ml, respectively. Our GC-MS analysis on the methanol extract resulted in the identification of 59 compounds for T. equestre. The prevalent compounds were 9,12- Octadecadienoic acid (Z,Z), Ergosta-5,8,22-trien-3-ol, (3.beta., 22E), Octadecanoic acid, 2,3-dihydroxypropyl ester, nHexadecanoic acid, 9,12-Octadecadienoic acid (Z,Z) -, 2-hydroxy-1-(hydroxymethyl) ethyl ester, Octadecanoic acid, 1- Dodecanol, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, n-Pentadecanol and Dodecane. Our study is the first report on the chemical composition of this wild mushroom.
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