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European journal of molecular biology and biochemistry

Volume 6, Issue 1, 2019
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
PROTECTIVE EFFECT OF SESAMOL ON ROTENONE-INDUCED C6 CELL LINE AND RAT BRAIN
Author
Rohini D and Vijayalakshmi K
Email
ammu.rohini333@gmail.com
keyword
Parkinson’s disease, Rotenone, Sesamol, C6 cell line, Brain.
Abstract
To investigate the protective effect of sesamol on rotenone-induced C6 cell line and rat brain. C6 cells were incubated with rotenone (20 μM) and sesamol at different concentrations (10-100 μM). Cell viability was determined by MTT assay. The reactive oxygen species, mitochondrial membrane potential and nuclear morphology were determined by dichlorofluorescein diacetate, rhodamine 123 and 4’, 6- diamidino -2- phenylindole, respectively. Experiments were carried out for 60 days in male Wistar albino rats (n= 6: I- vehicle control, II- rotenone (3 mg/kg.B.wt intraperitoneal), III- rotenone + sesamol (50 mg/kg.B.wt intraperitoneal), IV- rotenone + sesamol + L-DOPA (10 mg/kg.B.wt oral), V- rotenone + L-DOPA). Histopathology of the brain was assessed. Sesamol significantly increased the cell viability in rotenone-induced C6 cells. Sesamol reduced rotenone-induced reactive oxygen species generation, mitochondrial membrane potential impairment and nuclear damage. Histopathological evidences revealed that sesamol attenuated the injury caused by rotenone in the brain. The results obtained strongly indicate the promising role of sesamol against rotenone in both C6 cells and rat brain. Hence, sesamol can be helpful in the management of Parkinson’s disease treatment.
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