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American Journal of Biological and Pharmaceutical Research

Volume 7, Issue 2, 2020
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
ISOLATION OF POTENTIAL CELLULOLYTIC BACTERIUM, BACILLUS PUMILUS DGLB 32 FROM THE VEGETABLE WASTES OF DINDIGUL, SOUTH INDIA AND ITS GROWTH CHARACTERIZATION
Author
Esther Motaro* and T. Selvin Jebaraj Norman
Email
master.maniji@gmail.com
keyword
Bacillus pumilus; Dindigul; Cellulase; Production; Optimization.
Abstract
The present study aimed to isolate a promising cellulase producing bacterium from vegetable waste of Dindigul district, Tamil Nadu, India. This investigation isolated 34 morphologically distinct bacteria and only one strain that was DGLB32 showed potential cellulase production with 78.9 Units/ml. This strain was identified as Bacillus pumilus using 16s rRNA molecular sequencing method. The most potential strain showed maximum growth using the lab conditions of pH 7, 37°C temperature, 2% carboxymethylcellulose and 1% tannic acid. From the above observations, the isolated strain revealed the possibilities of industrial scale production especially for the production and applications of cellulase enzyme.
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