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European journal of molecular biology and biochemistry

Volume 6, Issue 1, 2019
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
PHYTOCHEMICAL ANALYSIS OF METHANOL EXTRACT OF OXYSTELMA ESCULENTUM R.BR.
Author
John Peter Paul J, Amster Regin Lawrence R, Sakunthala M and Muthupetchi S
Email
johnarock2008@yahoo.com
keyword
Oxystelma esculentum, Phytochemicals, UVVisible, HPLC, FTIR.
Abstract
The objective of the present study was to screen the phytochemicals present in the methanol extract of Oxystelma esculentum R.Br. The phytochemical screening of methanol extract was screened using the standard procedure for UV-Vis spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed the compounds separated at the nm of 250, 300, 350, 400, 450, 502, 534, 606, 664 and 700 with the absorption 1.699, 3.568, 3.505, 3.374, 0.867, 0.509, 0.423, 0.423, 1.001 and 0.018 respectively. The qualitative HPLC fingerprint profile displayed seven compounds at different retention times. The profile displayed four compounds at different retention times of 1.867min, 2.063min, 2.797min and 3.000min. The profile displayed three prominent peaks at the retention times of 1.687min, 2.063min and 2.797min, followed by only one moderate peak at the retention time of 3.000min. The result of FTIR analysis was found the presence of functional groups such as sulphonic acid, sulphates, cycloalkanes, phenyl, primary amines, methane, alkanes, aromatic, nitrate esters, 2-halo, alkanes and lactans.
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