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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
BIOSYNTHESIS OF SILVER NANOPARTICLES USING CENTELLA ASIATICA LEAVES EXTRACT AND ITS ANTIBACTERIAL ACTIVITY AGAINST CLINICAL PATHOGENS
Author
P.Srinivasan, A.Sengottaiyan, C.Sudhakar,P.Thiyagarajan, A.Kalirajan R.Thirumalaisamy and T.Selvankumar*
Email
t_selvankumar@yahoo.com
keyword
Centella asiatica, Aqueous extract, Silver nanoparticles, Pathogens, Antibacterial activity, Nanomedicine.
Abstract
In the present investigation, Ag NPs were synthesized using aqueous extract of Centella asiatica by simple and eco-friendly route. The aqueous silver ions when exposed to leaves extract of Centella asiatica were reduced and resulted in biosynthesis of Ag NPs. The Ag NPs were characterized by UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy–energy-dispersive spectroscopy (SEM-EDS). FTIR analysis was used to identify the functional groups in Ag NPs. The mean particle diameter of silver nanoparticle was calculated from the XRD pattern, according to the line width of the plane, and the refraction peak, using Scherer’s equation. SEM images revealed that the particles are spherical in nature. The EDS analysis of the Ag NPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver. The in vitro agar well diffusion method confirmed the potential antibacterial activity of the synthesized Ag NPs against Klebcella pneumonae and Streptococci aurous.
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