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Acta Biomedica Scientia

Volume 4, Issue 3, 2017
Mcmed International
Acta Biomedica Scientia
Issn
2348 - 215X (Print), 2348 - 2168 (Online)
Frequency
bi-annual
Email
editorabs@mcmed.us
Journal Home page
http://mcmed.us/journal/abs
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Abstract
Title
DEVELOPMENT AND VALIDATION OF FT-IR SPECTROMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF SERRATIOPEPTIDASE AND ROXITHROMYCIN IN BULK AND DOSAGE FORM
Author
Shirisha Vardhineedi*, Haribabu Y, Sheeja VK, Parasuraman R
Email
raamparasu7@gmail.com
keyword
Serratiopeptidase; Roxithromycin; KBr; FT-IR.
Abstract
A simple, economic, specific, accurate and precise validated FT-IR spectrometric method has been developed for the simultaneous estimation of Roxithromycin and Serratiopeptidase in bulk and dosage form. Here in present method spectrometry was carried out by using FT-IR spectrophotometer, Model FT/IR 4600 (JASCO). The linearity range for serratiopeptidase is 0.24-0.4mg and for roxithromycin 3.6-6mg. The selected spectral region is 1720-1740cm-1 and 1620-1680cm-1, related to a C=O stretch and C=C stretch bands of Roxithromycin and Serratiopeptidase respectively. The LOD and LOQ were 1.428mg and 4.328mg for Serratiopeptidase and 1.372mg and 4.159mg for Roxithromycin respectively. The percentage purity of Roxithromycin and Serratiopeptidase was found to be 97.02% and 92.79% respectively. This method was found to be accurate, precise, stable, robust and rugged as indicated by low values of %RSD. The developed method could be successfully applied for the simultaneous determination of Roxithromycin and Serratiopeptidase in combined pharmaceutical dosage form.
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