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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
EXTRACTION, ESTIMATION AND CHARACTERIZATION OF FUCOIDAN FROM PADINA TETRASTROMATICA HAUCK IN MANAPAD COAST, TAMIL NADU, INDIA.
Author
Shri Devi S.D.K.
Email
saishri28@yahoo.com
keyword
Seaweeds, Padina tetrastromatica, Fucoidan, UV-Vis, HPLC.
Abstract
The present study reports the estimation and characterization of fucoidan extracted from Padina tetrastromatica Hauck. collected from Manapad coast, the south east coast of Tamil Nadu, India. The fucoidan was isolated and estimated by Rioux method and screened by UV-Vis spectroscopic and HPLC analysis. The amount of fucoidan present in Padina tetrastromatica Hauck. is 19.60% of dry weight. The UV-Vis spectroscopic profile of the fucoidan extract of Padina tetrastromatica Hauck. was showed the compounds separated at 400nm, 450nm, 500nm, 550nm, 600nm, 669nm, 700nm, 750 and 800nm with the absorption, 0.414, 0.380, 0.244, 0.040, 0.065, 0.576, 0.032, 0.005 and 0.003 respectively. A sharp peak was observed at 669nm with the absorption 0.576. The qualitative HPLC fingerprint profile of the fucoidan extract of Padina tetrastromatica Hauck. was selected at a wavelength of 660nm due to the sharpness of the peaks and proper baseline. One prominent peak was observed and separated at the retention time of 1.500min. The profile displayed the screening and confirmation of fucoidan present in Padina tetrastromatica Hauck.
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