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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
SIMULTANEOUS ESTIMATION OF AZITHROMYCIN AND CEFIXIME IN BULK AND PHARMACEUTICAL FORMULATION BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author
Bala Rami Reddy Yenumula*, Mutta Reddy S, Bala Sekhara Reddy Ch
Email
drchalla2121@gmail.com
keyword
Azithromycin, Cefixime, HPLC, Methanol, Ortho Phospharic Acid.
Abstract
Azithromycin and Cefixime are both antibiotics drugs. As there is no UV or HPLC method for the simultaneous estimation of Azithromycin and Cefixime, a need was felt to develop the method for the analysis of both drugs simultaneously. This work concerns with the development and validation of a simple, specific and cost effective RP-HPLC method for simultaneous estimation of Azithromycin and Cefixime in bulk. In addition the developed method was applied to the suitable combined tablet dosage form ie., Zifi-AZ. Chromatography was carried on Kromasil C18 column with mobile phase comprising of Phosphate buffer (pH-5): Methanol: Acetonitrile (40:30:30, V/V/V). The flow rate was adjusted to 1ml/min with PDA detection at 260 nm. The retention times of Azithromycin and cefixime were found to be 2.8 min, 3.9 min respectively and other replicate standard system suitability parameters are within the limit and uniform. The different analytical parameters such as accuracy, precision, linearity, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined according to the International Conference on Harmonization (ICH) Q2B guidelines. The detector response was linear in the range of 0.5-1.5 mg/ml., 0.4-1.2 mg/ml for Azithromycin and cefixime respectively. The proposed method was successfully applied for the reliable quantification of active pharmaceuticals present in the commercial formulations.
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