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American Journal of Biological and Pharmaceutical Research

Volume 11, Issue 1, 2024
Mcmed International
American Journal of Biological and Pharmaceutical Research
Issn
2348 - 2176 (Print), 2348 - 2184 (Online)
Frequency
bi-annual
Email
editorajbpr@mcmed.us
Journal Home page
http://mcmed.us/journal/ajbpr
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Abstract
Title
COMPARISON OF THE QUALITY OF EXTENDED PHILIPPINE NATIVE CHICKEN (Gallus gallus domesticus) SPERM COLLECTED BY ABDOMINAL MASSAGE METHOD
Author
Heinz Kesniel D. Guieb*, Marcos B. Valdez and Flocerfida P. Aquino
Email
heinzniel12@gmail.com
keyword
Cryopreservation, semen extender, Cryobank.
Abstract
This study aimed to compare the use of two semen extenders after several hours of storage, i.e. 0h, 6h, 12h, 24h, 36h, 48h and 72h. Two chicken extenders were compared in the study: Lake’s Low Temperature (LLT) extender and AU extender. Semen was collected from six male chickens and was mixed with the extenders in a 1:1 ratio. Sperm motility, morphology and concentration were determined. Results showed that AU extender was able to produce viable sperms having a motility of > 30% after 24 h of storage at 5oC. On the other hand, LLT extended semen motility dropped to as low as 18% after 6 hours of storage at 5oC. However, both extenders were able to produce an acceptable range of normal sperm morphology (> 70%). The work conducted can be used for future studies for the cryopreservation of native chicken sperm and cryobanking purposes in the Philippines.
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