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European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
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Abstract
Title
MOLECULAR CHARACTERIZATION, OPTIMIZATION AND EFFICIENT PRODUCTION OF A BIOSURFACTANT FROM AN ESTUARINE YEAST CANDIDA TROPICALIS
Author
Boominathan M
Email
master.maniji@gmail.com
keyword
Production; Biosurfactant; Estuarine yeast; Candida tropicalis; Purification.
Abstract
A potential biosurfactant producing estuarine yeast Candida tropicalis was isolated from the mangrove sediment of Vellar Estuary, Porto Novo, Tamilnadu, India using multiple screening methods which showed appreciable results of β hemolytic activity, 2.7cm in oil displacement test, 72±4.3% activity in cell surface hydrophobicity and 55±1% emulsification activity. The potential yeast was identified as Candida tropicalis based on 18S rRNA sequencing method and blast homology search. The potential strain showed its enhanced production at 42nd h with the production medium supplemented with 2% of glucose, 1.5% of ammonium nitrate as carbon and nitrogen sources, ionic supplements of 0.3% MgSO4, 0.2% FeSO4, 0.1% of K2HPO4, ZnSO4 and CaCO3 along with the cultural conditions of 7 pH, 35°C temperature and 10pp salinity. Produced biosurfactant from the optimized conditions was isolated with acid precipitation method and purified using silica gel column chromatography. The final dry weight of the purified biosurfactant was accounted as 4.85g/L. The above findings show the possibilities of this biosurfactant production for industrial scale fermentation.
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