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Canadian Journal of Biological and Microbiology Research

Volume 2, Issue 1, 2016
Mcmed International
Canadian Journal of Biological and Microbiology Research
Issn
XXX-XXXX (Print), XXXX-XXXX (Online)
Frequency
bi-annual
Email
editorcjbmr@mcmed.us
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Abstract
Title
MULTIPLE RESISTANT ACTIVITIES AND MOLECULAR CHARACTERISATION OF Pseudomonas aeruginosa ISOLATED FROM TIRUPUR TEXTILE EFFLUENTS
Author
T.Jayaseelan, R.Damodaran, P. Mani*
Email
master.maniji@gmail.com
keyword
Textile effluent, P.aeruginosa Virulence factors, phylogenetic tree.
Abstract
A potential bacterial strains of Pseudomonas aeruginosa were isolated and selected from textile effluent discharged parts of Noyyal river, Tirupur, Tamilnadu, India. Virulence factors of P.aeruginosa were detected through protease, phospholipase and slime test. Strong slime production was found in two strains; higher protease (13 mm) was found in P2 and P3 strains; positive phospholipase activity were identified in 4 isolates of P.aeruginosa. Nucleotide sequences of multiple resistances P.aeruginosa were confirmed by 16S r DNA sequencing with universal bacterial primers (16S-UP-F, UP-R) which was deposited in Gene Bank (Accession Number KT175510), and phylogenetic tree was constructed. The sequences showed 100% similarity with the already established the species P.aeruginosa. From this study, the isolate was confirmed as multiple resistant P.aeruginosa which causes dangerous diseases to human beings and aquatic organisms.
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