TELL : +91 0 99 08 87 07 06

 Mcmed International ®: Largest Journal Publication in world

European journal of molecular biology and biochemistry

Volume 1, Issue 5, 2014
Mcmed International
European journal of molecular biology and biochemistry
Issn
2348 - 2192 (Print), 2348 - 2206 (Online)
Frequency
bi-annual
Email
editorejmbb@mcmed.us
Journal Home page
http://mcmed.us/journal/ejmbb
Recommend to
A Friend  | A librarian
Purchase
Purchase Issue
Abstract
Title
ASSOCIATION OF TOLL-LIKE RECEPTOR GENE POLYMORPHISM WITH SOMATIC CELL SCORE IN INDIGENOUS, CROSSBRED JERSEY AND CROSSBRED HOLSTEIN FRIESIAN CATTLE
Author
Haris Girish, S. N. Sivaselvam*, S. M. K. Karthickeyan and P. S. Rahumathulla
Email
snsivaselvam@tanuvas.org.in
keyword
TLR gene polymorphism, Somatic cell score, Resistance to mastitis, Cattle.
Abstract
Mastitis is a disease of major economic importance, causing reduced milk quality and leading to loss of milk production in cattle. The Toll-like receptors (TLRs) 2 and 4 play a role in the host response to inflammatory mastitis. The present investigation was carried out to study the polymorphism of TLR2 and TLR4 genes in cattle, to estimate the somatic cell count in milk and to study the relationship between TLR genes polymorphism and somatic cell score in mastitis. A total of 221 blood and milk samples were collected from 114 Jersey crossbred cows and Holstein Friesian crossbred cows suffering from mastitis and from 107 healthy indigenous, Jersey crossbred and Holstein Friesian crossbred cows with no previous history of mastitis. The PCR products were sequenced and screened for SNPs. There were 24 mutations (seven non-synonymous) in TLR2 gene and 11 mutations (one non-synonymous) in TLR4 gene. The SNPs detected were genotyped using both PCR-RFLP and tetra-primer ARMS-PCR. The mean SCC (x105/ml) was 2.31 ± 0.10 in normal milk samples and it was 68.85 ± 3.90 in mastitis samples. The SCS was 4.01 ± 0.031 and 9.43 ± 0.69 respectively. The most common pathogens found in the milk samples were Staphylococcus, E.coli, Streptococcus, Klebsiella and Pseudomonas. Samples harboring E.coli had the highest SCC (114.57± 4.2 x105/ml) when compared to others. The SNP 10095G>T of TLR2 gene influenced the SCS highly significantly. Among the three genotypes in 10095G>T, „GG‟ and „GT‟ genotypes were found to have lower mean SCS than the „TT‟ genotypes. Therefore, the GG genotypes would be resistant to mastitis.
Download Broucher
Back to Top >>>>